Protein Production and Processing in Young Adult and Aged Rat Submandibular Gland Cells In Vitro

Authors

B. J. Baum
Brian L. Kuyatt, Cedarville UniversityFollow
S. Humphreys

Document Type

Article

Publication Date

10-1983

Journal Title

Mechanisms of Ageing and Development

ISSN

0047-6374

Volume

23

Issue

2

First Page

123

Last Page

136

DOI

http://dx.doi.org/10.1016/0047-6374(83)90062-3

Abstract

Protein production and processing were evaluated in vitro in dispersed submandibular gland cells from young adult (4-6 months) and aged (24 month) rats. A modest decrease (approx. 25%) in protein production (incorporation of radiolabeled amino acids into 10% CCl3COOH-insoluble material) was found with old cells in both continuous-pulse and pulse-chase studies. Also new protein processing, followed by gel electrophoresis and autofluorography of radiolabeled samples, showed specific, marked alterations in old cells. In particular a significant difference in the processing of a 225 000 molecular weight glycoprotein (likely the major rat submandibular mucin) was detected.

Keywords

Exocrine, secretion, mucin, protein synthesis, salivary gland, submandibular gland, electrophoresis

PubMed ID

6656304

Recommended Citation

Baum, B. J.; Kuyatt, Brian L.; and Humphreys, S., "Protein Production and Processing in Young Adult and Aged Rat Submandibular Gland Cells In Vitro" (1983). Center for Teaching and Learning Publications. 11.
https://digitalcommons.cedarville.edu/ctl_publications/11