Title

Protein Production and Processing in Young Adult and Aged Rat Submandibular Gland Cells In Vitro

Document Type

Article

Publication Date

10-1983

Journal Title

Mechanisms of Ageing and Development

ISSN

0047-6374

Volume

23

Issue

2

First Page

123

Last Page

136

DOI

http://dx.doi.org/10.1016/0047-6374(83)90062-3

Abstract

Protein production and processing were evaluated in vitro in dispersed submandibular gland cells from young adult (4-6 months) and aged (24 month) rats. A modest decrease (approx. 25%) in protein production (incorporation of radiolabeled amino acids into 10% CCl3COOH-insoluble material) was found with old cells in both continuous-pulse and pulse-chase studies. Also new protein processing, followed by gel electrophoresis and autofluorography of radiolabeled samples, showed specific, marked alterations in old cells. In particular a significant difference in the processing of a 225 000 molecular weight glycoprotein (likely the major rat submandibular mucin) was detected.

Keywords

Exocrine, secretion, mucin, protein synthesis, salivary gland, submandibular gland, electrophoresis

PubMed ID

6656304