Title

Protein Production and Release by Dispersed Rat Submandibular Gland Cells In Vitro After Adrenergic Stimulation

Document Type

Article

Publication Date

9-14-1981

Journal Title

Life Sciences

ISSN

0024-3205

Volume

29

Issue

11

First Page

1143

Last Page

1151

DOI

http://dx.doi.org/10.1016/0024-3205(81)90203-4

Abstract

Enzymatically dispersed cell aggregates were prepared from rat submandibular glands. Cells were responsive to α- and β-adrenergic agonists, as measured by net K+ release and radiolabeled protein secretion, respectively. Protein production by submandibular gland cells was constant during the 90 min experimental period. Specific agonist and antagonist experiments demonstrated that both α- and β-adrenergic receptor stimulation were required for maximum secretion of newly synthesized protein. Proteins were radiolabeled with [35S] methionine and both soluble cell and secreted proteins examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autofluorography. A broad size range of newly synthesized proteins was detected (Mr∼104−5 × 105). Adrenergic stimulation (1-epinephrine) specifically increased the secretion of certain radiolabeled proteins and, in addition, resulted in both cellular and secreted proteins with electrophoretic characteristics distinct from that of control preparations.

Keywords

Rats, epinephrine, in vitro, proteins, stimulation, submandibular gland, sympathetic nervous system

PubMed ID

7289778