Title

Histochemical Evaluation of Thiamine Pyrophosphatase Activity During First Molar Odontogenesis of the Neonatal Hairless Mouse

Document Type

Article

Publication Date

1977

Journal Title

Acta Histochemica

ISSN

0065-1281

Volume

60

Issue

1

First Page

78

Last Page

90

DOI

http://dx.doi.org/10.1016/s0065-1281(77)80026-3

Abstract

Localization of thiamine pyrophosphatase activity has been evaluated in the developing first molar of the neonatal hairless mouse. Postnatal animals from parturitio to five days of age were decapitated and the severed heads frozen and sectioned in a frontal plane on a cryostat. 14 am thick sections were fixed and subsequently incubated for thiamine pyrophosphatase activity according to the method of Goldfischer et al. (1971). The tissue was visualized, dehydrated, cleared and mounted. Light microscopy was utilized in evaluating thiamine pyrophosphatase activity. Thiamine pyrophosphatase activity in the first molar of the hairless mouse is presented in tabular form and compared to similar data for the Swiss albino mouse. Enzyme activity increased as the metabolic activities of various cell layers increased. Thus, thiamine pyrophosphatase activity appeared to be related to the degree of differentiation and functional competency of the odontogenic tissues in the hairless mouse.

Keywords

Dental enamel, dental pulp, histocytochemistry, mice, odontoblasts, odontogenesis, thiamine pyrophosphatase

PubMed ID

413323

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