Pharmaceutical Sciences Faculty Publications

Title

Induction of Apoptosis in Fibroblasts by IL-1ß-Converting Enzyme, a Mammalian Homolog of the C. elegans Cell Death Gene ced-3

Document Type

Article

Publication Date

11-19-1993

Journal Title

Cell

Volume

75

Issue

4

First Page

653

Last Page

660

DOI

http://dx.doi.org/10.1016/0092-8674(93)90486-a

PubMed ID

8242741

Abstract

The mammalian interleukin-1β-converting enzyme (ICE) has sequence similarity to the C. elegans cell death gene ced-3. We show here that overexpression of the murine ICE (mICE) gene or of the C. elegans ced-3 gene causes Rat-1 cells to undergo programmed cell death. Point mutations in a region homologous between mICE and CED-3 eliminate the ability of mICE and ced-3 to cause cell death. The cell death caused by mICE can be suppressed by overexpression of the crmA gene, a specific inhibitor of ICE, as well as by bcl-2, a mammalian oncogene that can act to prevent programmed cell death. Our results suggest that ICE may function during mammalian development to cause programmed cell death.

Keywords

Apoptosis, fibroblasts, IL-1ß converting enzyme