Pharmacy Practice Faculty Publications

Title

Transport of L-glucose by Rhodotorula glutinis

Document Type

Article

Publication Date

2-1988

Journal Title

Biochimie

Volume

70

Issue

2

First Page

183

Last Page

185

DOI

10.1016/0300-9084(88)90059-4

Abstract

The kinetics of l-glucose transport by Rhodotorula glutinis were studied over a 720-fold range of sugar concentrations. Analysis of the saturation isotherm revealed the presence of a one-carrier system for l-glucose in the plasma membrane of Rhodotorula glutinis. This carrier exhibited a km of 3.7 ± 0.3 mM. d-Ribose was found to be a competitive inhibitor with a Ki of 19 ± 1 mM. The results suggest that l-glucose is transported by the high-Km, d-ribose carrier. l-Glucose was transported against a concentration gradient and the transport was inhibited by the proton conductor 2,4-dinitrophenol.

Keywords

L-gluclose, sugar, rhodotorula glutinis, active transport