Type of Submission

Poster

Keywords

Tetrahymena, netrin, tyrosine kinase

Abstract

Netrin 4 protein and its homologs are found throughout the animal kingdom. Netrin-4 is known to have a protective role against vascular damage. Previous studies have shown that human netrin-1 has a role in angiogenesis. This information about human netrin-1 and netrin-4 led us to research the pathway of netrin-4 in Tetrahymena thermophila. Our previous studies of the netrin proteins show that netrin-1 and netrin-3 are both repellents in Tetrahymena thermophila. The data in this study show that netrin-4 is also a repellent of Tetrahymena thermophila. These data suggest that netrin-4 could signal through the same pathway as netrin-1 and netrin-3. Furthermore, in our previous studies of the signaling pathways for netrin-1 and netrin-3, we discovered that netrin-1 signals through a tyrosine kinase pathway and netrin-3 signals through a serine/threonine kinase pathway. Because of this we decided to investigate whether or not netrin-4 signals through the same or different pathway as netrin-1 or netrin-3. We performed a cross adaptation assay on Tetrahymena thermophila using netrin-1, netrin-3, and netrin-4. The data from this assay suggests that netrin-4 does not signal through the same pathway as netrin-1 or netrin-3. Netrin-4 also was shown to have no effect on mitosis, which is similar to previous findings with netrin-1 in this organism. In contrast, netrin-3 peptides have been shown to inhibit mitosis in this organism.

Campus Venue

Stevens Student Center Lobby

Location

Cedarville, OH

Start Date

4-3-2019 11:00 AM

End Date

4-3-2019 2:00 PM

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Included in

Cell Biology Commons

Share

COinS
 
Apr 3rd, 11:00 AM Apr 3rd, 2:00 PM

Recombinant Netrin-4 Does Not Signal Through the Netrin-1 or Netrin-3 Pathway in Tetrahymena thermophila

Cedarville, OH

Netrin 4 protein and its homologs are found throughout the animal kingdom. Netrin-4 is known to have a protective role against vascular damage. Previous studies have shown that human netrin-1 has a role in angiogenesis. This information about human netrin-1 and netrin-4 led us to research the pathway of netrin-4 in Tetrahymena thermophila. Our previous studies of the netrin proteins show that netrin-1 and netrin-3 are both repellents in Tetrahymena thermophila. The data in this study show that netrin-4 is also a repellent of Tetrahymena thermophila. These data suggest that netrin-4 could signal through the same pathway as netrin-1 and netrin-3. Furthermore, in our previous studies of the signaling pathways for netrin-1 and netrin-3, we discovered that netrin-1 signals through a tyrosine kinase pathway and netrin-3 signals through a serine/threonine kinase pathway. Because of this we decided to investigate whether or not netrin-4 signals through the same or different pathway as netrin-1 or netrin-3. We performed a cross adaptation assay on Tetrahymena thermophila using netrin-1, netrin-3, and netrin-4. The data from this assay suggests that netrin-4 does not signal through the same pathway as netrin-1 or netrin-3. Netrin-4 also was shown to have no effect on mitosis, which is similar to previous findings with netrin-1 in this organism. In contrast, netrin-3 peptides have been shown to inhibit mitosis in this organism.

 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.