Chemorepellent Signaling Through the Lysozyme/PACAP Receptor Is Mediated Through cAMP and PKC in Tetrahymena thermophila

Authors

D. L. Hassenzahl
N. K. Yorgey
M. D. Keedy
A. R. Price
J. A. Hall
C. C. Myzcka
Heather G. Kuruvilla, Cedarville UniversityFollow

Document Type

Article

Publication Date

5-14-2001

Journal Title

Journal of Comparative Physiology A

Volume

187

Issue

3

First Page

171

Last Page

176

Abstract

Pituitary adenylate cyclase-activating peptide and lysozyme are potent chemorepellents which act through the same receptor in Tetrahymena. Using in vivo behavioral studies, we have found that the pituitary adenylate cyclase-activating peptide /lysozyme receptor appears to signal through a G-protein pathway which is mediated through both adenosine 3'5'monophosphate and protein kinase C. Avoidance to pituitary adenylate cyclase-activating peptide and lysozyme is inhibited by the G-protein inhibitor, guanosine 5'-O-(2-thiodiphosphate), the adenosine 3'5'monophate analog, Rp-adenosine-3', 5' cyclic monophosphorothioate, and the protein kinase C inhibitors, calphostin C and bisindolylmaleimide IV. A proposed model for signaling through the pituitary adenylate cyclase-activating peptide /lysozyme receptor is briefly outlined.

Keywords

Chemorepellent; PACAP-38; Tetrahymena; G-protein; PKC

Recommended Citation

Hassenzahl, D. L., Yorgey, N. K., Keedy, M. D. , Price, A. R. , Hall, J. A. , C.C. Myzcka, H.G. Kuruvilla, (2001). Chemorepellent signaling through the lysozyme/PACAP receptor is mediated through cAMP and PKC in Tetrahymena thermophila. Journal of Comparative Physiology A, 187, 171-176.