Science and Mathematics Faculty Publications

Title

Chemorepellent Signaling Through the Lysozyme/PACAP Receptor Is Mediated Through cAMP and PKC in Tetrahymena thermophila

Document Type

Article

Publication Date

5-14-2001

Journal Title

Journal of Comparative Physiology A

Volume

187

Issue

3

First Page

171

Last Page

176

Abstract

Pituitary adenylate cyclase-activating peptide and lysozyme are potent chemorepellents which act through the same receptor in Tetrahymena. Using in vivo behavioral studies, we have found that the pituitary adenylate cyclase-activating peptide /lysozyme receptor appears to signal through a G-protein pathway which is mediated through both adenosine 3'5'monophosphate and protein kinase C. Avoidance to pituitary adenylate cyclase-activating peptide and lysozyme is inhibited by the G-protein inhibitor, guanosine 5'-O-(2-thiodiphosphate), the adenosine 3'5'monophate analog, Rp-adenosine-3', 5' cyclic monophosphorothioate, and the protein kinase C inhibitors, calphostin C and bisindolylmaleimide IV. A proposed model for signaling through the pituitary adenylate cyclase-activating peptide /lysozyme receptor is briefly outlined.

Keywords

Chemorepellent; PACAP-38; Tetrahymena; G-protein; PKC

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