Rat Parotid Gland Amylase: Evidence for Alterations in an Exocrine Protein with Increased Age

Document Type

Article

Publication Date

5-1982

Journal Title

Mechanisms of Ageing and Development

ISSN

0047-6374

Volume

19

Issue

1

First Page

27

Last Page

35

DOI

http://dx.doi.org/10.1016/0047-6374(82)90047-1

Abstract

The content of alpha-amylase, the major exocrine secretory protein from rat parotid glands, was studied in young adult and aged rat tissue. alpha-Amylase protein was determined with an enzyme-linked immunosorbent assay. This employed antisera, produced against alpha-amylase purified from young adult rats, but which recognized and precipitated alpha-amylase enzyme activity equally well from both age groups. The parotid gland content of alpha-amylase was reduced about 50% in aged rats. Furthermore, the percentage of total gland protein which was alpha-amylase was decreased about 40% in aged animals. The data suggest that a somewhat specific alteration in alpha-amylase production (synthesis and/or degradation) occurs in parotid glands from aged rats. In addition, alpha-amylase functional activity was followed. The specific enzyme activity (U amylase activity per mg immunoreactive amylase) was about 35% higher in extracts from aged rat parotid glands compared to that of young adult glands.

Keywords

Aging, amylases, parotid gland, proteins, rats

PubMed ID

6180260

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