Pharmaceutical Sciences Faculty Publications

Quantitation of Monophosphorylated Lipid A in the Oil-in-Water Adjuvant Delivery Systems Using Transesterification and GC-MS.

Document Type

Article

Publication Date

7-1-2017

Journal Title

Journal of Pharmaceutical Sciences

ISSN

1520-6017

Volume

106

Issue

7

First Page

1760

Last Page

1763

DOI

10.1016/j.xphs.2017.02.034

PubMed ID

28285019

Abstract

Vaccine delivery systems play pivotal role in effective antigen delivery. These systems often contain adjuvants that stimulate specific immune response and are important for vaccines' efficacy and safety. Oil-in-water vaccine delivery lipid emulsion systems containing monophosphoryl lipid A (MPLA) as immune modulator have been extensively investigated in vaccine trials. Herein, we describe a simple orthogonal method, for quantitative measurement of MPLA in an oil-in-water lipid delivery system using direct transesterification reaction followed by gas-chromatography-mass spectrometry analysis. In this protocol, the transesterification reaction results in the release of fatty acid methyl esters followed by gas-chromatography-mass spectrometry-based targeted quantification of the specific 3-hydroxytetradecanoate fatty acid methyl ester to measure the concentration of MPLA in an oil-in-water lipid emulsion system.

Keywords

Adjuvants, Immunologic, Drug Delivery Systems, Emulsions, Esterification, Gas Chromatography-Mass Spectrometry, Lipid A, Oils, Pharmaceutical Vehicles, Vaccines, Water

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