Pharmaceutical Sciences Faculty Publications

Targeted Mass Spectrometry Approaches for Protein Biomarker Verification

Document Type

Article

Publication Date

11-18-2011

Journal Title

Journal of Proteomics

ISSN

1876-7737

Volume

74

Issue

12

First Page

2650

Last Page

2659

DOI

10.1016/j.jprot.2011.04.011

PubMed ID

21540133

Abstract

The search for protein biomarkers has been a highly pursued topic in the proteomics community in the last decade. This relentless search is due to the constant need for validated biomarkers that could facilitate disease risk stratification, disease diagnosis, prognosis, monitoring as well as drug development, which ultimately would improve our quality of life. The recent development of proteomic technologies including the advancement of mass spectrometers with high sensitivity and speed has greatly advanced the discovery of potential biomarkers. One of the bottlenecks lies in the development of well-established verification assays to screen the biomarker candidates identified in the discovery stage. Recently, absolute quantitation using multiple-reaction monitoring mass spectrometry (MRM-MS) in combination with isotope-labeled internal standards has been extensively investigated as a tool for high-throughput protein biomarker verification. In this review, we describe and discuss recent developments and applications of MRM-MS methods for biomarker verification.

Keywords

Biomarkers, drug design, mass spectrometry, proteome, proteomics

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