Pharmaceutical Sciences Faculty Publications

Plk1-Dependent and -Independent Roles of an ODF2 Splice Variant, hCenexin1, at the Centrosome of Somatic Cells

Document Type

Article

Publication Date

4-1-2009

Journal Title

Developmental Cell

ISSN

1878-1551

Volume

16

Issue

4

First Page

539

Last Page

550

DOI

10.1016/j.devcel.2009.02.004

PubMed ID

19386263

PubMed Central® ID

PMC2741019

Abstract

Outer dense fiber 2 (ODF2) was initially identified as a major component of the sperm tail cytoskeleton, and was later suggested to be localized to somatic centrosomes and required for the formation of primary cilia. Here we show that a splice variant of hODF2 called hCenexin1, but not hODF2 itself, efficiently localizes to somatic centrosomes via a variant-specific C-terminal extension and recruits Plk1 through a Cdc2-dependent phospho-S796 motif within the extension. This interaction and Plk1 activity were important for proper recruitment of pericentrin and gamma-tubulin, and, ultimately, for formation of normal bipolar spindles. Earlier in the cell cycle, hCenexin1, but again not hODF2, also contributed to centrosomal recruitment of ninein and primary cilia formation independent of Plk1 interaction. These findings provide a striking example of how a splice-generated C-terminal extension of a sperm tail-associating protein mediates unanticipated centrosomal events at distinct stages of the somatic cell cycle.

Keywords

Alternative splicing, amino acid sequence, antigens, centrosome, cilia, mitosis, biological, phosphorylation, phosphoserine, protein binding, tubulin

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