Pharmaceutical Sciences Faculty Publications

Title

Effect of Tumor Necrosis Factor-Alpha on Estrogen Metabolism and Endometrial Cells: Potential Physiological and Pathological Relevance

Document Type

Article

Publication Date

1-1-2009

Journal Title

The Journal of Clinical Endocrinology and Metabolism

ISSN

0021-972X

Volume

94

Issue

1

First Page

285

Last Page

293

DOI

10.1210/jc.2008-1389

PubMed ID

18957495

PubMed Central® ID

PMC2630861

Abstract

CONTEXT: Estrogen and its metabolites play a critical role in the pathophysiology of the endometrium. The bioavailability of estrogen and estrogen metabolites in endometrial tissues depends on the expression of enzymes involved in estrogen biosynthesis and metabolism. Substantial evidence indicates that estrogen-dependent endometrial disorders are also associated with proinflammatory milieu. However, the mechanism whereby inflammation contributes to these conditions is not known.

OBJECTIVE: The objective of the study was to investigate the effect of TNF-alpha on estrogen metabolism and the expression of estrogen-metabolizing genes in human endometrial glandular epithelial cells (EM1).

DESIGN: EM1 were treated with 17beta-estradiol (E2) with or without TNF-alpha. Capillary liquid chromatography-tandem mass spectrometry analysis was used for quantitative measurement of estrogens and estrogen metabolites. Western blot analysis, reporter gene assay, and real-time RT-PCR were used to assess the expression of estrogen-metabolizing genes.

RESULTS: TNF-alpha treatment significantly increased the level of total estrogen and estrogen metabolites and significantly increased the rate of conversion of estrone (E1) into E2. TNF-alpha also enhanced the oxidative metabolism of estrogen into catecholestrogens with concomitant inhibition of their conversion into methoxyestrogens. Gene expression analysis revealed that TNF-alpha induced the expression of genes involved in E2 biosynthesis (steroidogenic factor-1 and aromatase) and activation (17beta- hydroxysteroid dehydrogenase type 1 and cytochrome P-450, 1B1) with simultaneous repression of genes involved in estrogen inactivation (17beta-hydroxysteroid dehydrogenase type 2; catechol O-methyltransferase; and nicotinamide adenine dinucleotide phosphate-quinone oxidoreductase 1).

CONCLUSION: TNF-alpha increases the local estrogen biosynthesis in human endometrial glandular cells and directs estrogen metabolism into more hormonally active and carcinogenic metabolites. These effects may impact many physiological and pathological processes that occur within the endometrium.

Keywords

Aromatase, ell line, endometrium, estradiol, estradiol dehydrogenases, estrogens, gene expression regulation, steroidogenic factor 1, tumor necrosis factor-alpha

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