Pharmaceutical Sciences Faculty Publications

Title

Utility of separation science in metabolomic studies.

Document Type

Article

Publication Date

6-1-2008

Journal Title

Journal of Separation Science

ISSN

1615-9314

Volume

31

Issue

11

First Page

1936

Last Page

1947

DOI

10.1002/jssc.200700601

PubMed ID

18348322

Abstract

Metabolomics, or alternately metabonomics, an emerging field of biochemical research, is a complementary technique to genomics, transcriptomics, and proteomics. Direct quantitative measurements of metabolite expressions in urine, serum, plasma, and tissue are essential for the study of biological processes in normal and disease states. Since the number of metabolites in a biological sample is large separation science plays an important role in metabolomic research. This review describes the role of GC, HPLC, CE, and supercritical fluid chromatography in metabolomic research. The application of each technique is discussed and its advantages and limitations are discussed with selective illustrative examples.

Keywords

Animals, biomarkers, chemistry techniques, analytical, chromatography, humans, mass spectrometry, metabolism

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