Pharmaceutical Sciences Faculty Publications

Sample Preparation for Mass Spectrometry Analysis of Formalin-Fixed Paraffin-Embedded Tissue: Proteomic Analysis of Formalin-Fixed Tissue

Document Type

Article

Publication Date

1-1-2008

Journal Title

Methods in Molecular Biology

ISSN

1064-3745

Volume

425

First Page

131

Last Page

138

DOI

10.1007/978-1-60327-210-0_11

PubMed ID

18369892

Abstract

One of the great hopes in biomedical research is that proteomic technology can be used to identify novel biomarkers for diseases such as cancer. The challenge to discovering biomarkers starts with sample collection and continues right through data acquisition and bioinformatic analysis. Because the ultimate goal is to find indicators of human disease it is ideal to be able to study clinical samples. Unfortunately clinical samples such as serum, plasma, urine, and especially tissue biopsies are precious and are often difficult to obtain in sufficient quantities or numbers to conduct proteomic discovery studies. There exists, however, a vast archive of pathologically characterized clinical samples in the form of formalin fixed paraffin embedded tissue blocks. This chapter describes methods that have been developed to allow the proteins from these tissue samples to be excised in a form that is amenable for proteomic analysis by mass spectrometry.

Keywords

Chromatography, high pressure liquid, formaldehyde, paraffin embedding, proteomics, tandem mass spectrometry

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