Pharmaceutical Sciences Faculty Publications

Title

Enrichment of Low-Molecular-Weight Proteins from Biofluids for Biomarker Discovery

Document Type

Article

Publication Date

1-1-2005

Journal Title

Expert Review of Proteomics

ISSN

1744-8387

Volume

2

Issue

1

First Page

139

Last Page

145

DOI

10.1586/14789450.2.1.139

PubMed ID

15966859

Abstract

The dramatic progress in mass spectrometry-based methods of protein identification has triggered a new quest for disease-associated biomarkers. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and its variant surface-enhanced laser desorption/ionization mass spectrometry, provide effective means to explore the less studied information slice of the human serum proteome -- low-molecular-weight proteins and peptides. These low-molecular-weight proteins and peptides are promising for the detection of important biomarkers. Due to the significant experimental problems imposed by high-abundance and high-molecular-weight proteins, it is important to effectively remove these species prior to mass spectrometry analysis of the low-molecular-weight serum and plasma proteomes. In this review, the advantages afforded by recently introduced methods for prefractionation of serum, as they pertain to the detection and identification of biomarkers, will be discussed.

Keywords

Acetonitriles, biomarkers, blood proteins, chromatography, gel, ion exchange, electrophoresis, polyacrylamide gel, gene expression profiling, mass spectrometry, molecular weight, proteins, serum albumin, solvents, spectrometry, mass

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