Pharmaceutical Sciences Faculty Publications

Title

Characterization and Quantitation of Membrane Proteomes Using Multidimensional MS-Based Proteomic Technologies

Document Type

Article

Publication Date

8-1-2004

Journal Title

Expert Review of Proteomics

ISSN

1744-8387

Volume

1

Issue

2

First Page

153

Last Page

163

DOI

10.1586/14789450.1.2.153

PubMed ID

15966810

Abstract

A major goal of proteomics is to develop methods that enable the systematic characterization of every protein within the cell or particular subcellular proteome using a single analytical platform. Although the equivalent has already been achieved in genomics, reaching this goal in proteomics represents a much greater challenge due to the wide dynamic range of protein expression, numerous post-translational modifications and remarkable physicochemical heterogeneity of proteins. A major analytical challenge has involved developing more effective means for proteome-scale investigations of membrane proteins, whose solubility differs drastically from that of cytoplasmic proteins. Fortunately, rapid progress has increased the ability to characterize this critically important class of proteins on a scale analogous to that of aqueous soluble proteins.

Keywords

Cell adhesion, membrane proteins, molecular, protein conformation, proteins, proteomics, solubility, spectrometry, mass

Share

COinS