Characterization and Quantitation of Membrane Proteomes Using Multidimensional MS-Based Proteomic Technologies
Expert Review of Proteomics
A major goal of proteomics is to develop methods that enable the systematic characterization of every protein within the cell or particular subcellular proteome using a single analytical platform. Although the equivalent has already been achieved in genomics, reaching this goal in proteomics represents a much greater challenge due to the wide dynamic range of protein expression, numerous post-translational modifications and remarkable physicochemical heterogeneity of proteins. A major analytical challenge has involved developing more effective means for proteome-scale investigations of membrane proteins, whose solubility differs drastically from that of cytoplasmic proteins. Fortunately, rapid progress has increased the ability to characterize this critically important class of proteins on a scale analogous to that of aqueous soluble proteins.
Cell adhesion, membrane proteins, molecular, protein conformation, proteins, proteomics, solubility, spectrometry, mass
Blonder, Josip; Conrads, Thomas P.; and Veenstra, Timothy D., "Characterization and Quantitation of Membrane Proteomes Using Multidimensional MS-Based Proteomic Technologies" (2004). Pharmaceutical Sciences Faculty Publications. 459.