Coupling Morphogenesis to Mitotic Entry
Proceedings of the National Academy of Sciences of the United States of America
PubMed Central® ID
In eukaryotes, cyclin B-bound cyclin-dependent protein kinase 1 promotes mitotic entry but is held in check, in part, by Wee1 protein kinase. Timely mitotic entry in budding yeast requires inactivation of Swe1 (Wee1 ortholog). Perturbations of the septin collar at the bud neck lead to Swe1 stabilization, delaying the G(2)/M transition. Swe1 is recruited to the neck and hyperphosphorylated before ubiquitin-mediated degradation. Hsl1 kinase (Nim1 ortholog), a negative regulator of Wee1, is required for efficient Swe1 localization at the neck but seems not to phosphorylate Swe1. Here, we show that two other kinases targeted sequentially to the neck, Cla4/PAK and Cdc5/Polo, are responsible for stepwise phosphorylation and down-regulation of Swe1. This mechanism links assembly of a cellular structure to passage into mitosis.
Cell cycle proteins, mitosis, morphogenesis, phosphorylation, protein kinases, saccharomyces cerevisiae, proteins
Sakchaisri, Krisada; Asano, Satoshi; Yu, Li-Rong; Shulewitz, Mark J.; Park, Chong J.; Park, Jung-Eun; Cho, Young-Wook; Veenstra, Timothy D.; Thorner, Jeremy; and Lee, Kyung S., "Coupling Morphogenesis to Mitotic Entry" (2004). Pharmaceutical Sciences Faculty Publications. 480.