Pharmaceutical Sciences Faculty Publications
Coupling Morphogenesis to Mitotic Entry
Document Type
Article
Publication Date
3-23-2004
Journal Title
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424
Volume
101
Issue
12
First Page
4124
Last Page
4129
DOI
10.1073/pnas.0400641101
PubMed ID
15037762
PubMed Central® ID
PMC384705
Abstract
In eukaryotes, cyclin B-bound cyclin-dependent protein kinase 1 promotes mitotic entry but is held in check, in part, by Wee1 protein kinase. Timely mitotic entry in budding yeast requires inactivation of Swe1 (Wee1 ortholog). Perturbations of the septin collar at the bud neck lead to Swe1 stabilization, delaying the G(2)/M transition. Swe1 is recruited to the neck and hyperphosphorylated before ubiquitin-mediated degradation. Hsl1 kinase (Nim1 ortholog), a negative regulator of Wee1, is required for efficient Swe1 localization at the neck but seems not to phosphorylate Swe1. Here, we show that two other kinases targeted sequentially to the neck, Cla4/PAK and Cdc5/Polo, are responsible for stepwise phosphorylation and down-regulation of Swe1. This mechanism links assembly of a cellular structure to passage into mitosis.
Keywords
Cell cycle proteins, mitosis, morphogenesis, phosphorylation, protein kinases, saccharomyces cerevisiae, proteins
Recommended Citation
Sakchaisri, Krisada; Asano, Satoshi; Yu, Li-Rong; Shulewitz, Mark J.; Park, Chong J.; Park, Jung-Eun; Cho, Young-Wook; Veenstra, Timothy D.; Thorner, Jeremy; and Lee, Kyung S., "Coupling Morphogenesis to Mitotic Entry" (2004). Pharmaceutical Sciences Faculty Publications. 480.
https://digitalcommons.cedarville.edu/pharmaceutical_sciences_publications/480
