Pharmaceutical Sciences Faculty Publications

Title

Coupling Morphogenesis to Mitotic Entry

Document Type

Article

Publication Date

3-23-2004

Journal Title

Proceedings of the National Academy of Sciences of the United States of America

ISSN

0027-8424

Volume

101

Issue

12

First Page

4124

Last Page

4129

DOI

10.1073/pnas.0400641101

PubMed ID

15037762

PubMed Central® ID

PMC384705

Abstract

In eukaryotes, cyclin B-bound cyclin-dependent protein kinase 1 promotes mitotic entry but is held in check, in part, by Wee1 protein kinase. Timely mitotic entry in budding yeast requires inactivation of Swe1 (Wee1 ortholog). Perturbations of the septin collar at the bud neck lead to Swe1 stabilization, delaying the G(2)/M transition. Swe1 is recruited to the neck and hyperphosphorylated before ubiquitin-mediated degradation. Hsl1 kinase (Nim1 ortholog), a negative regulator of Wee1, is required for efficient Swe1 localization at the neck but seems not to phosphorylate Swe1. Here, we show that two other kinases targeted sequentially to the neck, Cla4/PAK and Cdc5/Polo, are responsible for stepwise phosphorylation and down-regulation of Swe1. This mechanism links assembly of a cellular structure to passage into mitosis.

Keywords

Cell cycle proteins, mitosis, morphogenesis, phosphorylation, protein kinases, saccharomyces cerevisiae, proteins

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