RNA-RNA Noncovalent Interactions Investigated by Microspray Ionization Mass Spectrometry
Rapid Communications in Mass Spectrometry
Electrospray ionization mass spectrometry is playing an increasing role in the study of noncovalent interactions involving biomolecules. RNA-RNA complexes are important in many areas of biology, including RNA catalysis, RNA splicing, ribosome function, and gene regulation. Here, microelectrospray mass spectrometry (microESI-MS) is used to study noncovalent base-pairing interactions between RNA oligonucleotides, an area not previously explored by this technique. Using a set of complementary RNA oligonucleotides, we demonstrate the formation of the expected double-helical RNA complexes composed of three distinct oligonucleotides. The ability to study specific RNA noncovalent interactions by microESI-MS has the potential to provide a unique method by which to analyze and assign precise molecular masses to RNA-RNA complexes.
Base sequence, molecular sequence data, oligonucleotides, protein binding, RNA, spectrometry, mass, electrospray ionization
Rodrigues Hoyne, P.; Benson, L. M.; Veenstra, Timothy D.; Maher, L. J.; and Naylor, S., "RNA-RNA Noncovalent Interactions Investigated by Microspray Ionization Mass Spectrometry" (2001). Pharmaceutical Sciences Faculty Publications. 512.