Pharmaceutical Sciences Faculty Publications

Effects of 1,25-Dihydroxyvitamin D3 on Growth of Mouse Neuroblastoma Cells

Document Type

Article

Publication Date

3-17-1997

Journal Title

Developmental Brain Research

ISSN

0165-3806

Volume

99

Issue

1

First Page

53

Last Page

60

DOI

10.1016/s0165-3806(96)00196-4

PubMed ID

9088565

Abstract

Epitopes of the 1,25-dihydroxyvitamin D(1,25(OH)2D3) receptor have been shown in developing dorsal root ganglia in fetal mice, as well as in cells maintained in culture [Johnson, J.A., Grande, J.P., Windebank, A.J. and Kumar, R., 1,25-Dihydroxyvitamin D3 receptors in developing dorsal root ganglia of fetal rats, Dev. Brain Res., 92 (1996) 120-124]. To investigate a possible role for 1,25(OH)2D3 in neural cell growth and development, a murine neuroblastoma cell line that expresses 1,25(OH)2D3 receptors, was treated with 1,25(OH)2D3. Treatment with 1,25(OH)2D3 resulted in a decrease in cell proliferation, a change in cell morphology, and the expression of protein markers of mature neuronal cells. The decrease in cell proliferation was accompanied by an increase in the expression of nerve growth factor (NGF). Anti-NGF monoclonal antibody added to the growth medium blocked the decrease in cell proliferation caused by 1,25(OH)2D3 treatment. Our results show that the sterol hormone 1,25(OH)2D3, causes a decrease in the proliferation of mouse neuroblastoma cells through alterations in the expression of NGF.

Keywords

Calcitriol, cell differentiation, cell division, mice, nerve growth factors, neuroblastoma, neurons, tumor cells, cultured

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