1,25-Dihydroxyvitamin D3 Regulates the Expression of N-myc, C-myc, Protein Kinase C, and Transforming Growth Factor-Beta2 in Neuroblastoma Cells
Biochemical and Biophysical Research Communications
1alpha,25-Dihydroxyvitamin D3 (1,25(OH)2D3) alters the proliferation of neuroblastoma cells in culture in part via a nerve growth factor (NGF)-mediated pathway. This suggests that factors other than NGF also play a role in the growth arrest induced by 1,25(OH)2D3. To more fully characterize the effect of 1,25(OH)2D3 on neuroblastoma cells, we treated the cells with 10(-8) M 1,25(OH)2D3 and examined the cells for changes in the expression of N-myc, c-myc, transforming growth factor-beta2 (TGF-beta2), and protein kinase C (PKC) activity. Our results show that 1,25(OH)2D3 causes a decrease in the expression of N-myc and c-myc, as well as a two-fold increase in total PKC activity and a dose-dependent increase in TGF-beta2 expression. These results show that 1,25(OH)2D3 regulates the expression of growth-regulatory factors other than NGF in neuroblastoma cells and that 1,25(OH)2D3 influences the growth of neural cells via multiple growth regulatory pathways.
Calcitriol, electrophoresis, polyacrylamide gel, gene expression regulation, genes, nerve growth factors, neuroblastoma, neurons, polymerase chain reaction, RNA, messenger, transforming growth factor beta, tumor cells, cultured
Veenstra, Timothy D.; Windebank, Anthony J.; and Kumar, Rajiv, "1,25-Dihydroxyvitamin D3 Regulates the Expression of N-myc, C-myc, Protein Kinase C, and Transforming Growth Factor-Beta2 in Neuroblastoma Cells" (1997). Pharmaceutical Sciences Faculty Publications. 542.