Elisha R. Injeti
Andrographolide, Carbamazepine, Cytochrome, Anticonvulsant, Herb-drug
Objective: To determine if andrographolide (AND) impacts the pharmacokinetics of carbamazepine (CBZ).
Background: CBZ is an anticonvulsant medication that is metabolized in the liver by cytochrome P450 (CYP) enzymes. AND is an over-the-counter medication that is common in Eastern cultures to treat inflammation and is a CYP enzyme inhibitor. Because CBZ is metabolized in the liver by these specific CYP enzymes, coadministration of andrographolide and CBZ could result in a herb-drug interaction.
Methods: Sprague-Dawley rats (N=12) aged between 3 months and 6 months (250-350 g) will be split into control (N=6) and treatment (N=6) groups. The treatment group will receive an AND injection (dissolved in dimethyl sulfoxide to 10 mg/mL) intraperitoneally for seven consecutive days. On the eighth day, another AND injection will be administered intraperitoneally as well as an injection of CBZ (CBZ powder prepared into a 20 mg/mL emulsion) administered via intravenous route. Plasma samples will be collected every 20 minutes for 4 hours and stored at -20℃.
Analysis: HPLC analysis will yield a time vs. plasma concentration graph that will allow us to calculate the rate of elimination (K). The mean K value will be determined for both the control and treatment group. The mean K value of 6 rats in the treatment group will be compared to the mean value of K of the 6 rats in the control group. These will be analyzed using SPSS and utilizing an unpaired t test, with a p
Aziz, Elizabeth; Franklin, Samuel; Pandav, Ankit; Savino, Abigail; Thompson, Caleb; VanDyke, Caleb; Choi, Ruth; and Injeti, Elisha R., "Herb-Drug Interaction of Andrographolide on the Pharmacokinetics of Carbamazepine in Rats" (2015). Pharmacy and Nursing Student Research and Evidence-Based Medicine Poster Session. 65.
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