Type of Submission

Poster

Keywords

Andrographolide, carbamazepine, cytochrome, anticonvulsant, herb-drug

Abstract

Objective: To determine if andrographolide (AND) impacts the pharmacokinetics of carbamazepine (CBZ).

Background: CBZ is an anticonvulsant medication that is metabolized in the liver by cytochrome P450 (CYP) enzymes. AND is an over-the-counter medication that is common in Eastern cultures to treat inflammation and is a CYP enzyme inhibitor. Because CBZ is metabolized in the liver by these specific CYP enzymes, coadministration of andrographolide and CBZ could result in a herb-drug interaction. Methods: Sprague-Dawley rats (N=12) aged between 3 months and 6 months (250-350 g) will be split into control (N=6) and treatment (N=6) groups. The treatment group will receive an AND injection (dissolved in dimethyl sulfoxide to 10 mg/mL) intraperitoneally for seven consecutive days. On the eighth day, another AND injection will be administered intraperitoneally as well as an injection of CBZ (CBZ powder prepared into a 20 mg/mL emulsion) administered via intravenous route. Plasma samples will be collected every 20 minutes for 4 hours and stored at -20℃.

Analysis: HPLC analysis will yield a time vs. plasma concentration graph that will allow us to calculate the rate of elimination (K). The mean K value will be determined for both the control and treatment group. The mean K value of 6 rats in the treatment group will be compared to the mean value of K of the 6 rats in the control group. These will be analyzed using SPSS and utilizing an unpaired t test, with a p<0.05 deemed statistically significant.

Campus Venue

Stevens Student Center

Location

Cedarville, OH

Start Date

4-20-2016 11:00 AM

End Date

4-20-2016 2:00 PM

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

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Apr 20th, 11:00 AM Apr 20th, 2:00 PM

The Effect of Andrographolide on the Metabolism of Carbamazepine in Rats

Cedarville, OH

Objective: To determine if andrographolide (AND) impacts the pharmacokinetics of carbamazepine (CBZ).

Background: CBZ is an anticonvulsant medication that is metabolized in the liver by cytochrome P450 (CYP) enzymes. AND is an over-the-counter medication that is common in Eastern cultures to treat inflammation and is a CYP enzyme inhibitor. Because CBZ is metabolized in the liver by these specific CYP enzymes, coadministration of andrographolide and CBZ could result in a herb-drug interaction. Methods: Sprague-Dawley rats (N=12) aged between 3 months and 6 months (250-350 g) will be split into control (N=6) and treatment (N=6) groups. The treatment group will receive an AND injection (dissolved in dimethyl sulfoxide to 10 mg/mL) intraperitoneally for seven consecutive days. On the eighth day, another AND injection will be administered intraperitoneally as well as an injection of CBZ (CBZ powder prepared into a 20 mg/mL emulsion) administered via intravenous route. Plasma samples will be collected every 20 minutes for 4 hours and stored at -20℃.

Analysis: HPLC analysis will yield a time vs. plasma concentration graph that will allow us to calculate the rate of elimination (K). The mean K value will be determined for both the control and treatment group. The mean K value of 6 rats in the treatment group will be compared to the mean value of K of the 6 rats in the control group. These will be analyzed using SPSS and utilizing an unpaired t test, with a p<0.05 deemed statistically significant.

 

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