Type of Submission

Poster

Keywords

E, E-farnesol, Burkholderia cepacia, biofilms, swarming motility, quorum sensing

Abstract

Burkholderia cepacia and Candida albicans both exhibit cell-to-cell communication through the use of quorum-sensing molecules (QSM) known as autoinducers. E,E-farnesol is a QSM produced by C. albicans which regulates its conversion from yeast to mycelium. Because there is a positive correlation between the presence of B. cepacia and C. albicans in the lungs of individuals with cystic fibrosis (CF), we examined whether E,E-farnesol had an effect on swarming motility in B. cepacia. Swarming motility was inhibited when B. cepacia was exposed to 250 µM of E,E-farnesol. In addition, there was a 26.8% decrease in rhamnolipid production when cells were grown in the presence of E,E-farnesol. These biosurfactants are known to regulate swarming motility. Changes in the rhamnoplipid concentrations could account for the inhibition of swarming motility observed in the presence of E,E-farnesol. The effect of E,E-farnesol on B. cepacia biofilms was also examined because these complex-community structures are detrimental to the lungs of CF patients and are quorum-sensing regulated. Crystal violet staining showed that E,E-farnesol did not significantly affect biofilm formation in B. cepacia. Further studies are needed to determine the effects of E,E-farnesol on established B. cepacia biofilms and whether it can be combined with traditional antibiotics to disrupt these structures.

Campus Venue

Stevens Student Center

Location

Cedarville, OH

Start Date

4-12-2017 11:00 AM

End Date

4-12-2017 2:00 PM

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

 
Apr 12th, 11:00 AM Apr 12th, 2:00 PM

E,E-farnesol Inhibits Swarming Motility in Burkholderia cepacia Through Rhamnolipid Production

Cedarville, OH

Burkholderia cepacia and Candida albicans both exhibit cell-to-cell communication through the use of quorum-sensing molecules (QSM) known as autoinducers. E,E-farnesol is a QSM produced by C. albicans which regulates its conversion from yeast to mycelium. Because there is a positive correlation between the presence of B. cepacia and C. albicans in the lungs of individuals with cystic fibrosis (CF), we examined whether E,E-farnesol had an effect on swarming motility in B. cepacia. Swarming motility was inhibited when B. cepacia was exposed to 250 µM of E,E-farnesol. In addition, there was a 26.8% decrease in rhamnolipid production when cells were grown in the presence of E,E-farnesol. These biosurfactants are known to regulate swarming motility. Changes in the rhamnoplipid concentrations could account for the inhibition of swarming motility observed in the presence of E,E-farnesol. The effect of E,E-farnesol on B. cepacia biofilms was also examined because these complex-community structures are detrimental to the lungs of CF patients and are quorum-sensing regulated. Crystal violet staining showed that E,E-farnesol did not significantly affect biofilm formation in B. cepacia. Further studies are needed to determine the effects of E,E-farnesol on established B. cepacia biofilms and whether it can be combined with traditional antibiotics to disrupt these structures.

 

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