Type of Submission

Poster

Keywords

Netrin, Western, Tetrahymena

Abstract

Netrin-3 is a guidance protein expressed throughout the animal kingdom, and involved in the development of branched structures such as the nervous system, lung, and mammary gland. We have previously shown that peptides derived from this protein serve as chemorepellents and mitotic inhibitors in Tetrahymena thermophila. Our previous work shows that Tetrahymena synthesize and secrete a netrin-3-like protein, as detected by ELISA. In this study, we find that a netrin-3-like protein is present in whole cell extract and secreted protein, as detected by Western blotting. A protein of approximately 48 kD is consistently detected in our Western blots. In addition, we often detect a protein of 52 kD, which may be the netrin-1-like protein of Tetrahymena that we have previously described. Further studies will enable us to determine whether the 52-kD protein is indeed the netrin-1 like protein of Tetrahymena.

Campus Venue

Stevens Student Center Lobby

Location

Cedarville, OH

Start Date

4-11-2018 11:00 AM

End Date

4-11-2018 2:00 PM

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

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Apr 11th, 11:00 AM Apr 11th, 2:00 PM

Netrin-3: Tracking the Elusive Antimitotic Signal on the Western Frontier

Cedarville, OH

Netrin-3 is a guidance protein expressed throughout the animal kingdom, and involved in the development of branched structures such as the nervous system, lung, and mammary gland. We have previously shown that peptides derived from this protein serve as chemorepellents and mitotic inhibitors in Tetrahymena thermophila. Our previous work shows that Tetrahymena synthesize and secrete a netrin-3-like protein, as detected by ELISA. In this study, we find that a netrin-3-like protein is present in whole cell extract and secreted protein, as detected by Western blotting. A protein of approximately 48 kD is consistently detected in our Western blots. In addition, we often detect a protein of 52 kD, which may be the netrin-1-like protein of Tetrahymena that we have previously described. Further studies will enable us to determine whether the 52-kD protein is indeed the netrin-1 like protein of Tetrahymena.

 

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