Science and Mathematics Faculty Publications

Title

PilJ Localizes to Cell Poles and Is Required for Type IV Pilus Extension in Pseudomonas aeruginosa

Document Type

Article

Publication Date

11-2007

Journal Title

Current Microbiology

ISSN

0343-8651

Volume

55

Issue

5

First Page

389

Last Page

395

DOI

10.1007/s00284-007-9008-5

Abstract

Twitching motility allows Pseudomonas aeruginosa to respond to stimuli by extending and retracting its type IV pili (TFP). PilJ is a protein necessary for this surface-associated twitching motility and bears high sequence identity with Escherichia coli methyl-accepting chemotaxis proteins (MCP). Here, we report that whereas wild-type P. aeruginosa PAO1 cells have extended pili at a single pole, pilJ mutant cells have shortened pili often at both poles despite normal levels of pilin accumulation, suggesting that PilJ is required for full TFP assembly/extension. Using yellow fluorescent protein fusions (pilJ-yfp), both plasmid born and in-frame chromosomal constructs, we determined that PilJ localizes to both poles of the cell. Overexpression of pilJ-yfp resulted in the protein accumulating between the poles.

Keywords

Pseudomonas, Twitching motility, Type IV pili

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

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