Chemorepellent Signaling Through the Lysozyme/PACAP Receptor Is Mediated Through cAMP and PKC in Tetrahymena thermophila
Journal of Comparative Physiology A
Pituitary adenylate cyclase-activating peptide and lysozyme are potent chemorepellents which act through the same receptor in Tetrahymena. Using in vivo behavioral studies, we have found that the pituitary adenylate cyclase-activating peptide /lysozyme receptor appears to signal through a G-protein pathway which is mediated through both adenosine 3'5'monophosphate and protein kinase C. Avoidance to pituitary adenylate cyclase-activating peptide and lysozyme is inhibited by the G-protein inhibitor, guanosine 5'-O-(2-thiodiphosphate), the adenosine 3'5'monophate analog, Rp-adenosine-3', 5' cyclic monophosphorothioate, and the protein kinase C inhibitors, calphostin C and bisindolylmaleimide IV. A proposed model for signaling through the pituitary adenylate cyclase-activating peptide /lysozyme receptor is briefly outlined.
Chemorepellent; PACAP-38; Tetrahymena; G-protein; PKC
Hassenzahl, D. L., Yorgey, N. K., Keedy, M. D. , Price, A. R. , Hall, J. A. , C.C. Myzcka, H.G. Kuruvilla, (2001). Chemorepellent signaling through the lysozyme/PACAP receptor is mediated through cAMP and PKC in Tetrahymena thermophila. Journal of Comparative Physiology A, 187, 171-176.