Pharmaceutical Sciences Faculty Publications

Preparation of the Low Molecular Weight Serum Proteome for Mass Spectrometry Analysis.

Document Type

Article

Publication Date

1-1-2019

Journal Title

Methods in Molecular Biology (Clifton, N.J.)

ISSN

1940-6029

Volume

2024

First Page

95

Last Page

102

DOI

10.1016/J.XPHS.2017.02.034

PubMed ID

31364044

Abstract

The ability to cure or manage many diseases is highly dependent on the ability to correctly diagnose them at the earliest possible stage. Diagnosis relies heavily on biomarkers whether these be visual symptoms or molecules found within samples acquired from the patient. For conditions that lack useful biomarkers, researchers are often faced with the task of sifting through very complex biological samples (i.e., serum, plasma, urine, tissue, cells, etc.) with the hope of discovering a small number of molecules that are exquisitely diagnostic for the condition of interest. One discovery strategy that has been frequently used is to fractionate the biological samples being studied into simpler aliquots that can be more easily characterized using existing technologies. One such fractionation method is to isolate a specific portion based on a specific property (i.e., size, phosphorylation state, charge, etc.) of the proteins within the sample. This method provides a simplified sample that can be characterized at a higher coverage level than the complex sample from which it was derived. This chapter details one of these methods, the extraction and analysis of the low molecular weight proteome of human serum.

Keywords

Biomarkers, low molecular weight proteome, mass spectrometry, protein depletion, serum

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