Pharmaceutical Sciences Faculty Publications

Quantitation of free and total bisphenol A in human urine using liquid chromatography-tandem mass spectrometry.

Document Type

Article

Publication Date

6-1-2011

Journal Title

Journal of Separation Science

ISSN

1615-9314

Volume

34

Issue

11

First Page

1268

Last Page

1274

DOI

10.1002/jssc.201100087

PubMed ID

21506272

Abstract

Bisphenol A (BPA) is employed in the synthesis of polycarbonate plastics and epoxy resins and is widely used in consumer products including as a coating for the inside of almost all food and beverage containers and thermal-imaging paper. Bisphenol A is considered to have important health implications because it possesses weak estrogenic activity and can leach from storage containers resulting in its consumption by both humans and animals. It is metabolized in the body and excreted into urine as a glucuronide derivative. In this report, we present an accurate, selective, sensitive, and reproducible high-performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS) method for the quantitation of BPA in human urine, which is not prone to exogenous contamination. BPA-glucuronide is hydrolyzed enzymatically, extracted with toluene, derivatized with dansyl chloride, and the BPA-(dansyl)(2) derivative is analyzed using reversed-phase HPLC/MS/MS. Calibration was linear to 50 ng/mL with a limit of quantitation of 50 pg/mL and a limit of detection of 5 pg/mL.

Keywords

Benzhydryl compounds, chromatography, molecular structure, phenols, tandem mass spectrometry

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