Pharmaceutical Sciences Faculty Publications
Clr4/Suv39 and RNA Quality Control Factors Cooperate to Trigger RNAi and Suppress Antisense RNA
Document Type
Article
Publication Date
3-25-2011
Journal Title
Science
ISSN
1095-9203
Volume
331
Issue
6024
First Page
1624
Last Page
1627
DOI
10.1126/science.1198712
PubMed ID
21436456
PubMed Central® ID
PMC6309853
Abstract
Pervasive transcription of eukaryotic genomes generates a plethora of noncoding RNAs. In fission yeast, the heterochromatin factor Clr4/Suv39 methyltransferase facilitates RNA interference (RNAi)-mediated processing of centromeric transcripts into small interfering RNAs (siRNAs). Clr4 also mediates degradation of antisense RNAs at euchromatic loci, but the underlying mechanism has remained elusive. We show that Clr4 and the RNAi effector RITS (RNA-induced transcriptional silencing) interact with Mlo3, a protein related to mRNA quality control and export factors. Loss of Clr4 impairs RITS interaction with Mlo3, which is required for centromeric siRNA production and antisense suppression. Mlo3 also interacts with the RNA surveillance factor TRAMP, which suppresses antisense RNAs targeted by Clr4 and RNAi. These findings link Clr4 to RNA quality control machinery and suggest a pathway for processing potentially deleterious RNAs through the coordinated actions of RNAi and other RNA processing activities.
Keywords
Cell cycle proteins, centromere, histones, mutation, RNA interference, RNA processing, RNA, antisense
Recommended Citation
Zhang, Ke; Fischer, Tamas; Porter, Rebecca L.; Dhakshnamoorthy, Jothy; Zofall, Martin; Zhou, Ming; Veenstra, Timothy D.; and Grewal, Shiv I. S., "Clr4/Suv39 and RNA Quality Control Factors Cooperate to Trigger RNAi and Suppress Antisense RNA" (2011). Pharmaceutical Sciences Faculty Publications. 241.
https://digitalcommons.cedarville.edu/pharmaceutical_sciences_publications/241
