Pharmaceutical Sciences Faculty Publications

Proteomic Identification of Binding Targets of Isothiocyanates: A Perspective on Techniques

Document Type

Article

Publication Date

6-10-2011

Journal Title

Journal of Proteomics

ISSN

1876-7737

Volume

74

Issue

7

First Page

1036

Last Page

1044

DOI

10.1016/j.jprot.2011.04.015

PubMed ID

21555001

PubMed Central® ID

PMC3107917

Abstract

Intake of cruciferous vegetable is inversely associated with the risk of several cancer types. Isothiocyanates (ITCs) are believed to be important constituents contributing to these cancer-preventive effects. Although several mechanisms, including induction of apoptosis, have been proposed for the anti-carcinogenesis activities of ITCs, detailed upstream triggering events are still not fully understood. Identification of ITC binding targets in cellular proteins is crucial for not only mechanistic studies but also future drug screening and design. In this review, we summarize recent progress in discovery of ITC protein targets from a technical perspective. The advantages and limitations of each method are discussed to facilitate future studies on target discovery of ITCs and perhaps other compounds.

Keywords

Anticarcinogenic agents, binding sites, chromatography, electrophoresis, gel, isothiocyanates, protein binding, proteomics, tandem mass spectrometry, tubulin

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