Comparison of Estrogens and Estrogen Metabolites in Human Breast Tissue and Urine

Authors

Emanuela Taioli
Annie Im
Xia Xu
Timothy D. Veenstra, Cedarville UniversityFollow
Gretchen Ahrendt
Seymour Garte

Document Type

Article

Publication Date

8-2-2010

Journal Title

Reproductive Biology and Endocrinology

ISSN

1477-7827

Volume

8

First Page

93

Last Page

93

DOI

10.1186/1477-7827-8-93

PubMed ID

20678202

PubMed Central® ID

PMC2922211

Abstract

BACKGROUND: An important aspect of the link between estrogen and breast cancer is whether urinary estrogen levels are representative of the intra-tissue levels of bioavailable estrogens.

METHODS: This study compares 15 estrogen and estrogen metabolite levels in breast tissue and urine of 9 women with primary breast cancer using a quantitative liquid chromatography-mass spectrometry method.

RESULTS: The average levels of estrogens (estrone, 17 beta-estradiol) were significantly higher in breast tissue than in urine. Both the 2 and the 16-hydroxylation pathways were less represented in breast tissue than urine; no components of the 4-hydroxypathway were detected in breast tissue, while 4-hydroxyestrone was measured in urine. However, the 2/16 ratio was similar in urine and breast tissue. Women carrying the variant CYP1B1 genotype (Leu/Val and Val/Val) showed significantly lower overall estrogen metabolite, estrogen, and 16-hydroxylation pathway levels in breast tissue in comparison to women carrying the wild type genotype. No effect of the CYP1B1 polymorphism was observed in urinary metabolites.

CONCLUSIONS: The urinary 2/16 ratio seems a good approximation of the ratio observed in breast tissue. Metabolic genes may have an important role in the estrogen metabolism locally in tissues where the gene is expressed, a role that is not readily observable when urinary measurements are performed.

Keywords

Breast neoplasms, carcinoma, ductal, estradiol, estrogens, female, gene frequency

Recommended Citation

Taioli, Emanuela; Im, Annie; Xu, Xia; Veenstra, Timothy D.; Ahrendt, Gretchen; and Garte, Seymour, "Comparison of Estrogens and Estrogen Metabolites in Human Breast Tissue and Urine" (2010). Pharmaceutical Sciences Faculty Publications. 266.
https://digitalcommons.cedarville.edu/pharmaceutical_sciences_publications/266