Pharmaceutical Sciences Faculty Publications

Characterization of the Human Ventricular Cerebrospinal Fluid Proteome Obtained From Hydrocephalic Patients

Document Type

Article

Publication Date

4-18-2010

Journal Title

Journal of Proteomics

ISSN

1876-7737

Volume

73

Issue

6

First Page

1156

Last Page

1162

DOI

10.1016/j.jprot.2010.02.004

PubMed ID

20176152

Abstract

The continuing expansion of proteomic technology has been fueled by the potential for discovering novel biomarkers that may be used for the early detection of disease. It has been proposed that human cerebrospinal fluid (CSF), which surrounds and protects the brain and spinal cord from traumatic injury, may be a valuable target for the diagnosis of a variety of conditions such as Alzheimer's disease, traumatic brain injury, amyotrophic lateral sclerosis and Parkinson's disease. The immense complexity of biofluids, however, still requires that considerable development be made in the analytical techniques used so that comprehensive coverage of the proteins present in such samples is achieved. Using a simple separation strategy the protein complement of human ventricular cerebrospinal fluid obtained from patients with hydrocephalus was evaluated. The study resulted in the identification of over 1500 unique proteins that were found within all nine CSF samples that were analyzed. Comparison with the HUPO serum proteome database demonstrated that human ventricular CSF contains a large array of proteins that may be unique to CSF. This analysis greatly increases our knowledge of the protein content of this clinically important biofluid.

Keywords

Algorithms, cations, cerebrospinal fluid proteins, chromatography, ion exchange, hydrocephalus, lipids, mass spectrometry, proteome, proteomics

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