Pharmaceutical Sciences Faculty Publications

Separation, Detection and Quantitation of Peptides by Liquid Chromatography and Capillary Electrochromatography

Document Type

Article

Publication Date

3-6-2009

Journal Title

Journal of Chromatography A

ISSN

1873-3778

Volume

1216

Issue

10

First Page

1825

Last Page

1837

DOI

10.1016/j.chroma.2008.12.052

PubMed ID

19131068

Abstract

This review discusses different liquid chromatographic and capillary electrochromatographic approaches to the separation and quantitation of peptides using silica-based and polymeric-based columns with emphasis on liquid chromatography. Mass spectrometry detection and quantitation of peptides using labeled and label-free procedures, will also be discussed, as well as the effect of amino acids' properties on the solubility of peptides, an important parameter that influences the selection of the mobile phase. A discussion of different column packing materials, reversed-phase, cyclodextrins, macrocyclic antibiotics, porous graphitic carbon, mixed-phases, and normal-phase will be included, as well as a short discussion of multi-dimensional approaches for the separation of complex peptide mixtures.

Keywords

Capillary electrochromatography, chromatography, high pressure liquid, mass spectrometry, peptides, polymers, proteomics, silicon dioxide

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