Pharmaceutical Sciences Faculty Publications

Quantitation of Steroid Hormones in Thin Fresh Frozen Tissue Sections

Document Type

Article

Publication Date

11-15-2008

Journal Title

Analytical Chemistry

ISSN

1520-6882

Volume

80

Issue

22

First Page

8845

Last Page

8852

PubMed ID

18937426

PubMed Central® ID

10.1021/ac801402a

Abstract

As analytical technologies in proteomics and metabolomics continue to mature, there is an increasing need to apply these to clinically relevant biologic samples. In this study, a liquid chromatography-tandem mass spectrometry method that utilizes selected reaction monitoring was used to measure the absolute quantity of estrogens and estrogen metabolites and testosterone in 8-microm tissue sections obtained from a fresh frozen lymph node tumor infiltrated by metastatic breast carcinoma. Total (conjugated plus unconjugated) and unconjugated levels of these steroid hormones were measured using two cohorts, each containing five adjacent serial sections cut from this tumor. The results were highly reproducible across replicate samples, showing that typical histological tissue sections represent an important sample type for the measurement of these specific metabolites.

Keywords

Cell line, tumor, chromatography, liquid, estrogens, frozen sections, hormones, humans, steroids, tandem mass spectrometry, testosterone

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