Pharmaceutical Sciences Faculty Publications

Clinical and Pharmaceutical Applications of Packed-Column Supercritical Fluid Chromatography

Document Type

Article

Publication Date

5-1-2008

Journal Title

Journal of Separation Science

ISSN

1615-9314

Volume

31

Issue

8

First Page

1223

Last Page

1230

DOI

10.1002/jssc.200700579

PubMed ID

18366028

Abstract

Packed-column supercritical fluid chromatography (pSFC) is a fast separation technique that combines the properties of HPLC and GC. pSFC with carbon dioxide as the mobile phase and packed silica column as the stationary phase possesses the properties of normal phase mechanism; however, the addition of modifiers to the mobile phase allows the separation of relatively polar compounds. In spite of its many positive attributes, pSFC has not been widely used in areas such as proteomics, where methods such as HPLC dominate. Packed column SFC has been extensively used in clinical and pharmaceutical laboratories, especially for separation of nonpolar and chiral drugs. This review will discuss recently published applications of pSFC, with a specific focus on its advantages and limitations for the analysis of pharmaceuticals with varying chemical properties.

Keywords

Biomarkers, chromatography, gas, high pressure liquid, supercritical fluid, ethanol, methanol, chemical, neoplasms, peptides

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