Pharmaceutical Sciences Faculty Publications

Proteomic Analysis of Protein Complexes

Document Type

Article

Publication Date

8-1-2007

Journal Title

Proteomics

ISSN

1615-9853

Volume

7

Issue

16

First Page

2688

Last Page

2697

DOI

10.1002/pmic.200700048

PubMed ID

17639601

Abstract

While it sometimes does not generate the publicity that biomarker discovery does, the identification of protein complexes may be the most popular use of proteomic technology. The partners a protein interacts with is a key component that describes the function of any protein. Basic research has long appreciated the importance of identifying protein interactions through techniques such as Western blotting and colocalization studies using immunofluorescence microscopy. This appreciation has carried over into the field of proteomics and brought with it the development of tools that increase the capabilities to characterize protein complexes to a far greater scale. While advances in technology have had a huge impact, sample preparation issues related to the isolation of protein complexes remains a critical factor in determining the success of these types of studies.

Keywords

Chromatography, liquid, electrophoresis, polyacrylamide gel, mass spectrometry, microscopy, fluorescence, proteins, proteomics

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