Pharmaceutical Sciences Faculty Publications
The Role of Electrophoresis in Disease Biomarker Discovery
Document Type
Article
Publication Date
6-1-2007
Journal Title
Electrophoresis
ISSN
0173-0835
Volume
28
Issue
12
First Page
1980
Last Page
1988
DOI
10.1002/elps.200600834
PubMed ID
17503404
Abstract
There has been increased activity in the last few years in the search for disease markers using fractionation of complex biological fluids combined with MS. While electrophoretic and chromatographic separations have played a major role in this endeavor, this manuscript is limited to a review of electrophoretic methods that have been established for disease biomarker discovery. These methods include 2-DE, difference gel electrophoresis (DIGE), and CE. We define what constitutes a biomarker, identify the steps required for establishing a biomarker, and describe the parameters needed in the design of an ideal diagnostic test. The application, advantages, and limitations of CE, DIGE, and 2-DE in meeting the goal of discovering novel biomarkers is discussed in detail, along with a few selected examples that illustrate the search for biomarkers for cancer and neurological diseases.
Keywords
Algorithms, biomarkers, tumor, blotting, body fluids, computational biology, electrophoresis, capillary, gel, mass spectrometry, neoplasms, neurodegenerative diseases, proteomics, urinalysis
Recommended Citation
Issaq, Haleem J. and Veenstra, Timothy D., "The Role of Electrophoresis in Disease Biomarker Discovery" (2007). Pharmaceutical Sciences Faculty Publications. 366.
https://digitalcommons.cedarville.edu/pharmaceutical_sciences_publications/366