Pharmaceutical Sciences Faculty Publications

Analysis of Fullerene-Based Nanomaterial in Serum Matrix by CE

Document Type

Article

Publication Date

5-1-2007

Journal Title

Electrophoresis

ISSN

0173-0835

Volume

28

Issue

10

First Page

1518

Last Page

1524

DOI

10.1002/elps.200600724

PubMed ID

17447247

Abstract

With the increasing interest in using nanoparticles as vehicles for drug delivery and image contrast agents, there is a need to develop assays for their detection and quantitation in complex matrices to facilitate monitoring their biodistribution. In this study, we developed a CE approach for the analysis of two nanoparticles: carboxyfullerene (C3) and dendrofullerene (DF1) in both standard solutions and a serum matrix. These highly soluble, charged C(60) derivatives were characterized by CZE using either a bare or dynamically coated fused-silica capillaries. The resolution of both nanoparticles was slightly lower with the coated capillary; however, their migration times were faster. While separation of the DF1 nanoparticles using MEKC resulted in a greater number of observable peaks, the peak profile of C3 was basically unchanged regardless of whether SDS micelles were added to the running buffers or not. The MEKC and/or CZE assays were then used to quantitate the C3 and DF1 nanoparticles in spiked human serum samples. The quantitation of the nanoparticles was linear from 0-500 microg/mL with detection limits ranging from 0.5 to 6 microg/mL.

Keywords

Buffers, calibration, chromatography, micellar electrokinetic capillary, electrophoresis, capillary, fullerenes, hydrogen-ion concentration, nanostructures, serum

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