Pharmaceutical Sciences Faculty Publications
Proteomic Profiling of Differentiating Osteoblasts
Document Type
Article
Publication Date
10-1-2006
Journal Title
Expert Review of Proteomics
ISSN
1744-8387
Volume
3
Issue
5
First Page
483
Last Page
496
DOI
10.1586/14789450.3.5.483
PubMed ID
17078763
Abstract
The major event that triggers osteogenesis is the transition of mesenchymal stem cells into bone-forming, differentiating osteoblast cells. Osteoblast differentiation is the primary event of bone formation, exemplified by the synthesis, deposition and mineralization of extracellular matrix. Osteoblast differentiation is controlled tightly by sequential activation of diverse transcription factors that regulate the expression of specific genes. The spatial and temporal regulation of the differentiation process is not completely understood at the cellular or molecular level. Recent advances in mass spectrometry-based proteomics have allowed for the systematic qualitative and quantitative profiling of differentiating osteoblasts, enabling a better understanding of the multiple factors and signaling events that control the differentiation process at a molecular level. This review focuses on recent developments in the proteomic analysis of differentiating osteoblasts, including advances, challenges and future prospects of using mass spectrometry to investigate the local and systemic factors regulating bone formation and its homeostasis.
Keywords
Animals, bone remodeling, cell differentiation, humans, osteoblasts, proteomics
Recommended Citation
Blonder, Josip; Xiao, Zhen; and Veenstra, Timothy D., "Proteomic Profiling of Differentiating Osteoblasts" (2006). Pharmaceutical Sciences Faculty Publications. 382.
https://digitalcommons.cedarville.edu/pharmaceutical_sciences_publications/382