Pharmaceutical Sciences Faculty Publications

Title

Proteomic Profiling of Differentiating Osteoblasts

Document Type

Article

Publication Date

10-1-2006

Journal Title

Expert Review of Proteomics

ISSN

1744-8387

Volume

3

Issue

5

First Page

483

Last Page

496

DOI

10.1586/14789450.3.5.483

PubMed ID

17078763

Abstract

The major event that triggers osteogenesis is the transition of mesenchymal stem cells into bone-forming, differentiating osteoblast cells. Osteoblast differentiation is the primary event of bone formation, exemplified by the synthesis, deposition and mineralization of extracellular matrix. Osteoblast differentiation is controlled tightly by sequential activation of diverse transcription factors that regulate the expression of specific genes. The spatial and temporal regulation of the differentiation process is not completely understood at the cellular or molecular level. Recent advances in mass spectrometry-based proteomics have allowed for the systematic qualitative and quantitative profiling of differentiating osteoblasts, enabling a better understanding of the multiple factors and signaling events that control the differentiation process at a molecular level. This review focuses on recent developments in the proteomic analysis of differentiating osteoblasts, including advances, challenges and future prospects of using mass spectrometry to investigate the local and systemic factors regulating bone formation and its homeostasis.

Keywords

Animals, bone remodeling, cell differentiation, humans, osteoblasts, proteomics

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