Pharmaceutical Sciences Faculty Publications

Quantitative Proteomic Analysis of Human Breast Epithelial Cells with Differential Telomere Length

Document Type

Article

Publication Date

5-18-2007

Journal Title

Biochemical and Biophysical Research Communications

ISSN

0006-291X

Volume

356

Issue

4

First Page

942

Last Page

947

DOI

10.1016/j.bbrc.2007.03.069

PubMed ID

17395154

PubMed Central® ID

PMC2268026

Abstract

Telomeres play important functional roles in cell proliferation, cell cycle regulation, and genetic stability, in which telomere length is critical. In this study, quantitative proteome comparisons for the human breast epithelial cells with short and long telomeres (184-hTERTL vs. 184-hTERTS and 90P-hTERTL vs. 90P-hTERTS), resulting from transfection of the human telomerase reverse transcriptase (hTERT) gene, were performed using cleavable isotope-coded affinity tags. More than 2000 proteins were quantified in each comparative experiment, with approximately 77% of the proteins identified in both analyses. In the cells with long telomeres, significant and consistent alterations were observed in metabolism (amino acid, nucleotide, and lipid metabolism), genetic information transmission (transcription and translation regulation, spliceosome and ribosome complexes), and cell signaling. Interestingly, the DNA excision repair pathway is enhanced, while integrin and its ligands are downregulated in the cells with long telomeres. These results may provide valuable information related to telomere functions.

Keywords

Breast, cell line, DNA repair, epithelial cells, proteome, telomere

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