Pharmaceutical Sciences Faculty Publications
Analysis of Fifteen Estrogen Metabolites Using Packed Column Supercritical Fluid Chromatography-Mass Spectrometry
Document Type
Article
Publication Date
3-1-2006
Journal Title
Analytical Chemistry
ISSN
0003-2700
Volume
78
Issue
5
First Page
1553
Last Page
1558
DOI
10.1021/ac051425c
PubMed ID
16503607
Abstract
Packed column supercritical fluid chromatography with tandem mass spectrometry was used for the separation of estrone, estradiol, estriol, 16-epiestriol, 17-epiestriol, 16-ketoestradiol, 16alpha-hydroxyestrone, 2-methoxyestrone, 4-methoxyestrone, 2-hydroxyestrone-3-methyl ether, 2-methoxyestradiol, 4-methoxyestradiol, 2-hydroxyestrone, 4-hydroxyestrone, and 2-hydroxyestradiol. A gradient of methanol in carbon dioxide (0-30% methanol in 15 min, 2% change/min) at a flow rate of 2 mL/min and cyanopropyl silica column connected in series with a diol column, both 2.1 mm i.d. x 150 mm long, packed with 5-mum spherical silica-based particles, resulted in the separation and quantification of all 15 estrogens in less than 10 min. The limit of detection (LOD) and limit of quantitation (LOQ) of this pSFC MS/MS method was determined to be 0.5 (S/N = 3), and 5 pg, respectively. Compared with RP-HPLC MS analysis of the same mixture in terms of speed of analysis and sensitivity, pSFC MS is much faster, 10 versus 70 min, with comparable LOD and LOQ.
Keywords
Carbon dioxide, chromatography, supercritical fluid, estrogens, metabolism, methanol, sensitivity, specificity, tandem mass spectrometry
Recommended Citation
Xu, Xia; Roman, John M.; Veenstra, Timothy D.; Van Anda, Jennifer; Ziegler, Regina G.; and Issaq, Haleem J., "Analysis of Fifteen Estrogen Metabolites Using Packed Column Supercritical Fluid Chromatography-Mass Spectrometry" (2006). Pharmaceutical Sciences Faculty Publications. 410.
https://digitalcommons.cedarville.edu/pharmaceutical_sciences_publications/410