Pharmaceutical Sciences Faculty Publications
Investigation of the Mouse Serum Proteome
Document Type
Article
Publication Date
9-1-2005
Journal Title
Journal of Proteome Research
ISSN
1535-3893
Volume
4
Issue
5
First Page
1561
Last Page
1568
DOI
10.1021/pr050107r
PubMed ID
16212407
Abstract
With the rapid assimilation of genomic information and the equally impressive developments in the field of proteomics, there is an unprecedented interest in biomarker discovery. Although human biofluids represent increasingly attractive samples from which new and more accurate disease biomarkers may be found, the intrinsic person-to-person variability in these samples complicates their discovery. One of the most extensively used animal models for studying human disease is mouse because, unlike humans, they represent a highly controllable experimental model system. Unfortunately, very little is known about the proteomic composition of mouse serum. In this study, a multidimensional fractionation approach on both the protein and the peptide level that does not require depletion of highly abundant serum proteins was combined with tandem mass spectrometry to characterize proteins within mouse serum. Over 12 300 unique peptides that originate from 4567 unique proteins-approximately 16% of all known mouse proteins-were identified. The results presented here represent the broadest proteome coverage in mouse serum and provide a foundation from which quantitative comparisons can be made in this important animal model.
Keywords
Amino acid sequence, blood proteins, cations, chromatography, liquid, computational biology, mass spectrometry, peptides, protein, proteome, proteomics, sequence homology, amino acid, trypsin
Recommended Citation
Hood, Brian L.; Zhou, Ming; Chan, King C.; Lucas, David A.; Kim, Grace J.; Issaq, Haleem J.; Veenstra, Timothy D.; and Conrads, Thomas P., "Investigation of the Mouse Serum Proteome" (2005). Pharmaceutical Sciences Faculty Publications. 435.
https://digitalcommons.cedarville.edu/pharmaceutical_sciences_publications/435