Pharmaceutical Sciences Faculty Publications
Profiling of Secreted Proteins from Human Ovarian Cancer Cell Lines by Surface-Enhanced Laser Desorption Ionization Time-of-Flight Mass Spectrometry
Document Type
Article
Publication Date
8-2003
Journal Title
Journal of Liquid Chromatography & Related Technologies
ISSN
1082-6076
Volume
26
Issue
14
First Page
2315
Last Page
2328
DOI
10.1081/JLC-120023249
Abstract
Surface‐enhanced laser desorption ionization (SELDI) time‐of‐flight mass spectrometry (TOF MS) was used to profile six human ovarian cancer cell lines. Non‐confluent cell cultures were exchanged into serum free medium and allowed to grow for either 24 or 48 hours, at which time the medium was collected and analyzed using a Ciphergen SELDI‐TOF MS and a QSTAR Pulsar QqTOF mass spectrometer fitted with a SELDI source. The spectra showed the presence of several low molecular weight species, as well as differences and similarities in the proteins detected in the media of the six ovarian tumor cell lines. The same species were detected at 24 and 48 hours and reproducible changes in their relative abundances were observed.
Keywords
Biomarker detection, protein profile, cancer
Recommended Citation
Prieto, DaRue; Conrads, Thomas P.; Scudiero, Dominic A.; Veenstra, Timothy D.; and Issaq, Haleem J., "Profiling of Secreted Proteins from Human Ovarian Cancer Cell Lines by Surface-Enhanced Laser Desorption Ionization Time-of-Flight Mass Spectrometry" (2003). Pharmaceutical Sciences Faculty Publications. 486.
https://digitalcommons.cedarville.edu/pharmaceutical_sciences_publications/486
