Quantitative Proteomics Employing Primary Amine Affinity Tags

Authors

Van M. Hoang
Thomas P. Conrads
Timothy D. Veenstra, Cedarville UniversityFollow
Josip Blonder
Atsushi Terunuma
Jonathan C. Vogel
Robert J. Fisher

Document Type

Article

Publication Date

9-1-2003

Journal Title

Journal of Biomolecular Techniques

ISSN

1524-0215

Volume

14

Issue

3

First Page

216

Last Page

223

PubMed ID

13678152

PubMed Central® ID

PMC2279946

Abstract

A proteomics-based method using stable isotope labeling to assess the relative abundance of peptides or proteins is described. Bradykinin and carbonic anhydrase were labeled with sulfosuccinimidyl-2-(biotinamido) ethyl-1,3-dithiopropionate, a membrane impermeant reagent that is reactive with primary amines. Specificity of the label to primary amines was demonstrated using tandem mass spectrometry. Also, relative quantitation was achieved by secondary labeling with natural isotopic abundance and stable isotope-labeled methyl iodide. We believe this to be an effective stable isotope-labeling method for quantitative proteomics.

Keywords

Amines, amino acid sequence, carbonic anhydrases, lysine, proteomics, spectrometry, mass

Recommended Citation

Hoang, Van M.; Conrads, Thomas P.; Veenstra, Timothy D.; Blonder, Josip; Terunuma, Atsushi; Vogel, Jonathan C.; and Fisher, Robert J., "Quantitative Proteomics Employing Primary Amine Affinity Tags" (2003). Pharmaceutical Sciences Faculty Publications. 488.
https://digitalcommons.cedarville.edu/pharmaceutical_sciences_publications/488