Pharmaceutical Sciences Faculty Publications
Methods for Fractionation, Separation and Profiling of Proteins and Peptides
Document Type
Article
Publication Date
9-1-2002
Journal Title
Electrophoresis
ISSN
0173-0835
Volume
23
Issue
17
First Page
3048
Last Page
3061
DOI
10.1002/1522-2683(200209)23:17<3048::AID-ELPS3048>3.0.CO;2-L
PubMed ID
12207315
Abstract
In the last few years there has been an increased effort to develop technologies capable of identifying and quantifying large numbers of proteins expressed within a cell system (i.e., the proteome). The complexity of the mixtures being analyzed has made the development of effective fractionation and separation methods a critical component of this effort. This review highlights many of the protein and peptide fractionation and separation methods, such as electrophoresis and high-performance liquid chromatography (HPLC), which have experienced significant development over the past forty years. Modern instrumental strategies for the resolution of cell proteins, based on separations employing a single high-resolution or multidimensional approach, and the relative merits of each, will be discussed. The focus of this manuscript will be on the development of multidimensional separations such as two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), HPLC/HPLC, and HPLC-capillary electrophoresis and their application to the characterization of complex proteome mixtures.
Keywords
Chromatography, electrophoresis, peptides, proteins
Recommended Citation
Issaq, Haleem J.; Conrads, Thomas P.; Janini, George M.; and Veenstra, Timothy D., "Methods for Fractionation, Separation and Profiling of Proteins and Peptides" (2002). Pharmaceutical Sciences Faculty Publications. 500.
https://digitalcommons.cedarville.edu/pharmaceutical_sciences_publications/500
