Pharmaceutical Sciences Faculty Publications

A Sheathless Nanoflow Electrospray Interface for On-Line Capillary Electrophoresis Mass Spectrometry

Document Type

Article

Publication Date

4-1-2003

Journal Title

Analytical Chemistry

ISSN

0003-2700

Volume

75

Issue

7

First Page

1615

Last Page

1619

DOI

10.1021/ac020661+

PubMed ID

12705593

Abstract

A novel, rugged capillary electrophoresis-electrospray ionization (CE-ESI) interface where the separation column, an electrical porous junction, and the spray tip are integrated on a single piece of a fused-silica capillary is described. ESI is accomplished by applying an electrical potential through an easily prepared porous junction across a 3-4-mm length of fused silica. A stable electrospray is produced at nanoflow rates generated in the capillary by electrophoretic and electroosmotic forces. The interface is particularly well suited for the detection of low-femtomole levels of proteins and peptides. The ruggedness of this interface was evident by the continuous operation of the same column for over a 2-week period with no detectable deterioration in separation or electrospray performance. The new interface was used for the LC-ESI-MS separation and analysis of peptides and proteins. Injection of 25 fmol of [Glu1]-fibrinopeptide B using the new device produced a CE-ESI-MS electropherogram with a signal-to-noise ratio of over 100 for this peptide.

Keywords

Amino acid sequence, apoproteins, chromatography, liquid, electrophoresis, capillary, horses, myoglobin, nanotechnology, proteins, spectrometry, mass, electrospray ionization

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