Pharmaceutical Sciences Faculty Publications

Distribution of 1,25-Dihydroxyvitamin D3 Receptor Immunoreactivity in the Rat Brain and Spinal Cord

Document Type

Article

Publication Date

2-1-1999

Journal Title

Journal of Chemical Neuroanatomy

ISSN

0891-0618

Volume

16

Issue

2

First Page

135

Last Page

145

DOI

10.1016/s0891-0618(99)00002-2

PubMed ID

10223312

Abstract

A complete mapping study on the 1,25-dihydroxyvitamin D3 receptor immunoreactivity within the rat central nervous system was performed with a monoclonal and a polyclonal antibody. Specific immunostaining was observed within both nuclear and cytoplasmic compartments of a variety of cells in the cerebellum, mesopontine area, diencephalon, cortex, spinal cord, and limbic system. Both monoclonal and polyclonal antibodies provided similar staining patterns. The monoclonal antibody stained distinct domains within the nuclei of all and the cytoplasm of specific neuronal cell types, like motor neurons, Purkinje cells, and pyramidal cells of the cortex more clearly than the polyclonal antibody. The expression of vitamin D3 receptor in the rat central nervous system was confirmed by in situ hybridisation. The widespread distribution of vitamin D3 receptor in distinct portions of the sensory, motor, and limbic brain systems suggests multiple functional properties of 1,25-dihydroxyvitamin D3 in the central nervous system.

Keywords

Animals, brain chemistry, immunohistochemistry, rats, Sprague-Dawley, receptors, calcitriol, spinal cord

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